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KMID : 0381219800120030169
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Journal of RIMSK
1980 Volume.12 No. 3 p.169 ~ p.186
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Study on the Fractions and Amount of Sialoglycoprotein in Gastric Mucosa of the Chronic Peptic Ulcer Patient
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Abstract
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Although the peptic ulcers are considered as an isolated disease entity which could occur at the site, where gastric juice could attack, and which could not differentiated according to its sites of origin, according to recent studies, there are some differencies in the pathogenesis of acute and chronic peptic ulcers, and gastric and duodenal chronic peptic ulcers. The characteristics of acute peptic ulcer, which could be evoked by most animal experiments and is a main patterns of stress ulcer of man, could be summerized as acute onset, multiplicities of the lesions, morphologically erosions, and no correlationships with sites of epithelial transitions. Against such characteristics, the chronic ulcers of the human beings occur insidiously as localized lesion at the site of epithelial transitions and frequently accompanied with chronic atrophic gastritis, and it is morphologically classic ulcer, but not erosion.
Moreover, some differencies in pathogenesis and clinical manifestation are well-documented between chronic peptic ulcers of stomach and duodenum. In chronic peptic ulcer of the duodenum, the patients have usually high secretory activities of gastric juice, high acidities of gastric juice, hyperreactive response to stimulants, excess drive to secrete gastric juice, and defects in inhibitory action to the gastric section.
The gastric ulcer patients are characterized by antral stasis and its related hypergastrinemia, H+ ion back-diffusion due to defects in the defense barrier of the gastric wall.
In some gastric ulcer patients, there are considerable evidences suggesting bile regurgitati on.
In ture, the duodenal ulcer is characteried by activation of attack force of gastric juice and the gastric ulcer is characterized by weakness or defects of defense mechanism.
In order to confirm such differencies in defense mechanism between the duodenal and gastric ulcer, the authors collected gastric specimens, whose were diagnosed as gastric carcinoma (40 cases), gastric chronic ulcer (27 cases) and chronic duodenal ulcer (10 cases) at the Dept. of Clinical Pathology, Chung-Ang University, Medical School Hospital from Nov. 1977 to Dec. 1979. The gastric specimens were maped, dissected, homogenated, and centrifuged at 20, 000 G for 20 minutes with phosphate buffer, pH 7. 4. The supernatents and sediments were examined with Kinsbery-Clark´s method, phenol-sulfuric method, modified Winzler´s method, and thiobarbituric method to estimate the amounts of protein, protein-bound carbohydrate, seromucoid, and sialic acid.
The following results were ensued;
1. There was decrease in the amounts of protein in the gastric mucosal supernatent of the ulcerative region of the gastric ulcer (56. 0±29. 79mg/100ml/g) and increased of the cancerous region of the gastric cancer (84.0±47.64mg/100m1/g) than that of the duodenal ulcer (74. 2±46.83mg/l00gr/g).
2. There was increase in protein amounts of the gastric mucosal precipitant of the noncancerous region of the gastric cancer (76.0±90.32mg/100ml/g) than that of duodenal ulcer (44.1-35.61mg/100ml/g). There were no significant changes of the protein amounts in the cancerous region of the gastric cancer, and ulcerative and nonulcerative region of the gastric ulcer.
3. There was significant increase in the protein-bound-carbohydrate amount in the cancerous region of the gastric cancer (3.6±2.67mg/100ml/g) than that of duodenal ulcer (1. 8±1. 26mg /100ml/g). There were some increasing tendencies in that of noncancerous region of the gastric cancer and ulcerative and nonulcerative regions of the gastrice ulcer.
4. There were no significant differences in the amounts of protein-bound-carbohydrate of the gastric mucosal precipitant between the examined groups.
5. There were signicant decrease in amounts of seromucoid in the gastric mucosal supernatent of the ulcerative region of the gastric ulcer (280.7±88. 47mg/100ml/9), of the nonulcerative region of the gastric ulcer(323.4±143.87mg/100ml/g), and noncancerous region of the gastric cancer (217.6±111.83mg/100ml/g) than that of duodenal ulcer (450.9± 175.15mg/100ml/g).
6. There were no significant differences in the seromucoid amounts of the gastric mucosal precipitant according location in the gestric mucosa of the duodenal ulcer patients and noncancerous region of the gastric cancer patients.
However, in the gastric ulcer, the least one was estimated from the gastric mucosal supernatent of the nonulcerated incisural region, wherewas the most predilection site of chronic peptic ulcer of the stomach during this study.
7. Although there is no significant changes of the sialic acid amounts of the supernatent and sediment of the gastric mucosa amoung the examined groups, there were some trend of decrease in the gastric ulcer mucosa.
8. The coefficiency of variation of the data for seromucoid, estimated in the gastric mucosa, was 38.8%.
9. There were some suspicious correlation between the amounts of seromucoid in the gastric mucosal supernatent and pathogenesis of the chronic peptic ulcer of the stomach.
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